Synthesis of a peptide nucleic acid oligomer with pyrimido[4,5-d]pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone as a nucleobase

Intraperitoneal Infection Infection: Proof of Principle Using Mouse as a Therapeutic Strategy against Bacterial Peptide Nucleic Acid Antisense Oligomer

Peptide nucleic acid antisense oligomer as a therapeutic strategy against bacterial infection: proof of principle using mouse intraperitoneal infection.

Antisense oligodeoxynucleotides (ODNs) and their analogs have been successfully utilized to inhibit gene expression and bacterial growth in vitro or in cell culture. In this study, acpP-targeting antisense peptide nucleic acid (PNA) and its peptide conjugate were tested as potential antibacterial agents in two groups of experiments using a mouse model. In the first group, Escherichia coli mutan...

Cellular Morphology and Immunologic Properties of Escherichia coli Treated With Antimicrobial Antisense Peptide Nucleic Acid

  Background & Objectives: Antisense peptide nucleic acids (PNA) that target growth essential genes show potent bactericidal properties without cell lysis. We considered the possibility that whether PNA treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to Dendritic cells (DCs). DCs are the most potent antigen presenting cells in th...

Synthesis of nucleobase-caged peptide nucleic acids having improved photochemical properties.

A nucleobase-caged peptide nucleic acid (PNA) having a (6-bromo-7-methoxycoumarin)-4-ylmethoxycarbonyl (Bmcmoc) caging group was newly synthesized. The Bmcmoc-caged PNAs were photolyzed to produce parent PNAs with a high photochemical efficiency. Introduction of a single Bmcmoc group was sufficient to suppress polymerase chain reaction (PCR) clamping activity and triplex invasion complex format...

A Review of Peptide Nucleic Acid

Abbreviations: DNA: Deoxyribonucleic Acid; RNA: Ribonucleic Acid; PNA: Peptide Nucleic Acid; WC: Watson-Crick; HG: Hoongsteen; NMM: N-Methyl Morpholine; DSC: Differential Scanning Calorimetry; RP-HPLC: Reverse Phase High Performance Liquid Chromatography; DsRed: Red Fluorescent Protein; SNPs: Single Nucleotide Polymorphisms; ACE: Affinity Capillary Electrophoresis; PNA-FISH: PNA Fluorescence In...